ABSTRACT
A pentaplex PCR was developed and optimised to detect the genes that encode the five most important putative virulence factors in Aeromonas isolates. It seems to be more efficient than previously reported techniques and promises to be a powerful tool for more accurate risk assessments and for monitoring pathogenic strains.
Subject(s)
Humans , Adhesins, Bacterial/analysis , Aeromonas/isolation & purification , Bacterial Infections , Diagnostic Techniques and Procedures , Enzyme Activation , Genetic Techniques , In Vitro Techniques , Polymerase Chain Reaction/methods , Virulence Factors , Methods , Patients , Methods , VirulenceABSTRACT
The isolation of Aeromonas bestiarum from common carps (Cyprinus carpio L.), cultivated at Santa Maria Chapa de Mota, is reported for the first time. The genetic identification for differentiating A. bestiarum from A. salmonicida is here emphasized.
Se informa por primera ocasión el aislamiento de Aeromonas bestiarum a partir de carpa común (Cyprinus carpio L.) de cultivo, procedente de Santa María Chapa de Mota, Estado de México, México. Se enfatiza la identificación genética para la diferenciación entre A. salmonicida y A. bestiarum.